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Future, we handled cells with distinctive concentrations of NAD+. When additional for twenty-four h at eighty µM in HeLa and 400 µM in U2OS cells, NAD+ impaired the incorporation of EdU (Fig. 2a and Prolonged Info Fig. 1k,l). Furthermore, sixteen µM of NAD+ was ample to scale back fork pace by ~forty% in HeLa cells without having influen